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KMID : 1140120070120030174
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Cancer Prevention Research
2007 Volume.12 No. 3 p.174 ~ p.185
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Application of Comet-Fluorescence in situ Hybridization (FISH) Technology in Cancer Prevention Study
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Park Eun-Ju
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Abstract
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The Comet assay (single cell gel electrophoresis) is a simple method for detecting DNA single- and double-strand breaks in eukaryotic cell. It has become a standard technology for the measurement of oxidative DNA damage both in vitro and in vivo. Research on DNA damage induced by carcinogens or repaired by bioactive components from vegetables and fruits has been performed during the last years with the Comet assay. In 1997, a new exciting approach has been introduced by Mckelvey-Martin etal. adapting the fluorescent in situ hybridization (FISH) to the Comet assay methodology to allow simultaneous detection of overall DNA damage and DNA breaks in specific regions of the genome, such as oncogene, tumor suppressor gene. Although the application of Comet-FISH to cancer prevention study is still in it’s infancy, it will be a good addition to the current existing tests for cancer prevention studies, because this technique allows rapid and easy screening of DNA breakage sensitivity after exposure to various kinds of carcinogens or anticarcinogens from in vitro study to human intervention study. (Cancer Prev Res 12, 174-186, 2007)
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KEYWORD
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Comet-FISH, Cancer prevention, DNA damage
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